UNIFI Training Event
From 7 to 12 July 2025, the Department of Biology at the University of Florence (UNIFI), Italy, hosted an intensive BGE training event focused on whole-genome and barcode sequencing using Pacific Biosciences (PacBio) platforms. The training formed part of Work Package 12 (Joint Network Activities), under Task T12.1 (Joint Network Training), and was delivered at the Florence Genomics Facility, which is equipped with first-, second-, and third-generation sequencing platforms and associated infrastructure for high-throughput library preparation.
The aim of the training was to provide early-career researchers and technical personnel with both theoretical background and hands-on experience in DNA extraction, library preparation, and sequencing workflows. The programme emphasized the practical implementation of established protocols for de novo genome sequencing and multiplexed barcode library preparation, particularly in cases involving diverse biological materials.
Due to the technical complexity and time required for sequencing system setup and library construction, the training was designed for a small, focused group. A total of five trainees participated: three technical staff from the University of Primorska (Slovenia) and two from the University of Florence. Over the course of five days, participants engaged in lectures, practical labs, and live demonstrations of sequencing runs using the PacBio Sequel IIe platform and SMRTbell prep kit 3.0.
The training covered the full workflow from high-quality DNA extraction to barcode and whole-genome library construction, quality control, and sequencing. Labs were structured to facilitate active, hands-on learning under direct supervision from instructors, enabling participants to apply techniques and troubleshoot in real time. The event was designed to empower participating teams to independently prepare sequencing-ready libraries either in-house or for submission to external service providers.
This capacity-building activity supported BGE’s goals of enabling high-quality sequencing at scale and expanding technical expertise across European biodiversity research institutions.